Anders Lunde

• Lunde, Anders & Glover, Joel Clinton (2020). A versatile toolbox for semi-automatic cell-by-cell object-based colocalization analysis. Scientific Reports. doi: 10.1038/s41598-020-75835-7. Fulltekst i vitenarkiv
• Lunde, Anders; Okaty, Benjamin W; Dymecki, Susan M & Glover, Joel Clinton (2019). Molecular Profiling Defines Evolutionarily Conserved Transcription Factor Signatures of Major Vestibulospinal Neuron Groups. eNeuro. doi: 10.1523/ENEURO.0475-18.2019. Fulltekst i vitenarkiv

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• Bochynska, Agata; Christiansen, Torgeir; Lunde, Anders & Bergsaker, Anne Schad (2024). Infrastructures for storing and sharing data. Fulltekst i vitenarkiv
• Glover, Joel Clinton & Lunde, Anders (2019). Transcription factor expression patterns in developing vestibulospinal neurons. Universitetet i Oslo. Fulltekst i vitenarkiv Vis sammendrag

  There is a group of special neurons in the brainstem called vestibulospinal (VS) neurons that are important for the control of balance and posture of all vertebrate animals, including humans. The VS neurons receive direct sensory information from the vestibular part of the inner ear. This informs them about the current spatial orientations and movements of the head. As animals are exposed to unexpected movements and changes in posture, the VS neurons can through a quick reflex help activate spinal cord motoneurons and muscle contraction. This VS reflex is important for the maintenance of a stable and upright posture. To learn more about the mechanisms during embryonic development that leads to the generation of these unique neurons in mouse and chicken embryos, this thesis work focuses on characterizing the gene expression patterns of developing VS neurons, with an emphasis on transcription factors. By using fluorescent retrograde tracing, RNA sequencing, immunohistofluorescence, high-throughput imaging, and a custom developed microscopy image colocalization analysis tool, several expression patterns are detailed and put into an evolutionary context.

• Lunde, Anders (2012). Expression of the bacterial ectA gene in the chloroplast of Chlamydomonas reinhardtii. Universitetet i Oslo. Fulltekst i vitenarkiv Vis sammendrag

  Ectoine is a small molecule that confers salt tolerance to organisms. It is synthesized in bacteria by the gene products of ectA, ectB, and ectC. Ectoine is used in medical, biotechnological, and cosmetic products. The purpose of this project was to determine whether expression of the ectA gene in the chloroplast of the model alga Chlamydomonas reinhardtii would be sufficient for ectoine production. Non-photosynthetic Chlamydomonas was transformed by microprojectile bombardment, using a plasmid vector with a chimeric ectA gene and a photosynthesis selection marker. In addition, a transformation vector with a codon optimized version of the ectA gene was designed, and used for bombardment. Transformants were analyzed by DNA and RNA blotting techniques. SDS-PAGE, and mass spectrometry were used to evaluate EctA accumulation. HPLC, and salt tolerance experiments were used to evaluate the accumulation of ectoine. One chloroplast transformant was isolated for the non-optimized ectA gene that was about fifty percent homoplasmic. No transformant could be isolated with the codon optimized ectA. Transcript accumulation was detected for the ectA transformant, however at low levels. EctA protein accumulation could not be detected. HPLC did not identify any ectoine accumulation, and no increased salt tolerance of the ectA transformant was observed. It is concluded that the current chimeric ectA gene is insufficient in supporting high levels of ectoine accumulation, probably because of the low transcript levels supported, and the lack of codon optimization. Further work is needed to improve the accumulation of EctA to higher levels.

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